Optical mapping in cardiac tissue: Advances and applications (Video) Event as iCalendar

(Seminars)

22 March 2016

4 - 5pm

Venue: Ground Floor Seminar Room (G10)

Location: 70 Symonds Street, Auckland Central.

Portrait of Dr Richard Walton

A talk from Dr. Richard Walton, LIRYC institute - University of Bordeaux, France.

Abstract

Optical mapping utilises fluorescent dyes sensitive to changes of transmembrane potential or concentrations of specific ions to measure spatio-temporal dynamics of electrophysiological behavior. The basic elements of an optical mapping setup includes excitation light sources, such as lasers or LEDs, and photon detectors fitted with filters appropriate to the emission spectra of dyes of interest. Due to the diffusive nature of light, optical signals are not purely surface signals, but originate from a sub-surface volume. Conventionally, excitation light in the blue/green visible spectra is utilised, which provides signals limited to activity near to the imaged surface. However, advances towards near-infrared dyes have enabled increased depth penetration in to myocardium due to reduced scattering and absorption of longer wavelength light. Here we discuss the utility of near infrared potentiometric dyes for the development towards three-dimensional reconstruction of electrical activity. In addition, the application of optical mapping to investigate electrophysiological heterogeneities and the mechanisms of arrhythmias will be discussed.

Biography

I started my undergraduate in Physiology at the University of Leeds in 2002, which I completed with honors in 2005. Following an experimental project in cardiology in my final year, I continued in the cardiac field with a PhD under the supervision of Dr Matthew Lancaster entitled: Ageing and exercise in the ventricular myocardium. I was introduced to optical mapping during my first Post Doc with Dr Olivier Bernus from 2008. Olivier continues to supervise me in Bordeaux, France at the LIRYC institute since 2011 where I have held both a french national foundation funded research fellow and Marie Curie research fellow positions.