Keith Pereira

Keith Pereira

ME student
BE(Hons)

Contact details

Uniservices House,
Room 439 630
70 Symonds St, Level 6
Auckland

Phone: +64 9 923 9341; internal ext 89341
Email: kper053@aucklanduni.ac.nz

Biography

I am a biomedical engineering student with a keen interest in the field of tissue engineering and regenerative medicine that was initially developed during my Part IV Engineering Project. This interest was further developed while working at Mesynthes (now called Aroa Biosurgery), a company with a primary focus in regenerative medicine and wound healing. I have a Bachelor of Engineering (Honours) in Biomedical Engineering from the University of Auckland, and commenced my masters in 2015.

Thesis

3D Traction Force Microscopy for endothelial cells under flow conditions

Cells are the building blocks that define living organisms. Cells can build complex organizations by proliferating, mechanically interacting with each other, and actively responding to their (micro) environment. For example, endothelial cell adhesion and migration is a critical step in many processes, including angiogenesis. Endothelial cell traction forces play an important in all these processes.

Two common methods of cellular traction force measurement include: micro-particle tracking of micron-sized exogenous particles in the cytoplasm; and traction force microscopy. The first method involves injecting fluorescent particles into living cells, applying a known force to the cells and then tracking the resulting displacement of each flourescent particle. The second method involves culturing cells on an extracellular matrix (e.g., polyacrylamide gels) embedded with fluorescent particles. The displacement of these particles due to cellular traction forces near the surface can be measured and analysed to estimate cellular traction forces using traction force microscopy.

The aims of this project are to construct a cell culture flow system and then perform 3D traction force microscopy on a cultured immortalized line of human microvascular endothelial cells (HMEC-1).
 

Supervisor

Dr David Long