Auckland Bioengineering Institute


Work-loop calorimeter

bioinst-jc-han
June-Chiew Han using the work-loop calorimeter.

The ABI Bioinstrumentation Laboratory has developed the world’s only flow-through calorimeter for measuring simultaneously the mechanical (force, length) and energetic (heat production) properties of realistically-contracting individual heart muscles. With this device, we can programmatically vary the amount of work done by a living heart muscle while measuring its energy consumption.

Our device combines sensitive temperature sensors with a sophisticated laser-interferometer based force-length control system. We electrically stimulate the muscle, causing it to contract and describe a force-length “work-loop” modelled on the pressure-volume behaviour of the whole heart. We measure the rate at which heat is produced by the muscle to 100 nW resolution while controlling muscle length to 2 nm resolution and force to 1 μN resolution.

This unique instrument is being used by our “experimental cardiac energetics” researchers in several major studies:

  • Dr June-Chiew Han is using this instrument in an HRC-funded study of the effect of diabetes on cardiac efficiency.
  • Dr Kenneth Tran is collecting data on effect of hypertension on cardiac efficiency for an NIH-funded study.
  • Dr Nari Kim is developing multi-scale mathematical models of heart tissue using data measured from this instrument.
  • Toan Pham is studying the underlying causes of cardiac dysfunction associated with cardiac hypertrophy.


On this page

Project members, collaborators and contributors


Instrumentation Leader

Portrait of

Andrew Taberner
Associate Professor
Email: a.taberner@auckland.ac.nz

Portrait of

June-Chiew Han
Research Fellow
Email: j.han@auckland.ac.nz

Portrait of

Kimberley Mellor
Honorary Academic
Email: k.mellor@auckland.ac.nz

Nari Kim

Nari Kim
Doctoral Candidate
Email: nari.kim@auckland.ac.nz

 

Scientific Leader

Portrait of

Denis Loiselle
Associate Professor
Email: ds.loiselle@auckland.ac.nz

Portrait of

Kenneth Tran
Research Fellow
Email: k.tran@auckland.ac.nz

Contributors

Soyeon Goo (PhD) 2009 to 2014
Measuring the effect of omega-3 fatty acids on heart muscle efficiency

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Postgraduate opportunities


For postgraduate opportunities that suit your expertise/interest, please contact:

Portrait of

June-Chiew Han
Research Fellow
Email: j.han@auckland.ac.nz

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Funding partners


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Publications


  1. An innovative work-loop calorimeter for in vitro measurement of the mechanics and energetics of working cardiac trabeculae
    Taberner, A.J., Han, J.C., Loiselle, D.S., and Nielsen, P.M.
    Journal of Applied Physiology, 2011. 10.1152/japplphysiol.00752.2011
  2. A unique micromechanocalorimeter for simultaneous measurement of heat rate and force production of cardiac trabeculae carneae
    Han, J.C., Taberner, A.J., Kirton, R.S., Nielsen, P.M., Smith, N.P., and Loiselle, D.S.
    Journal of Applied Physiology 2009. 107(3): p. 946-951. 10.1152/japplphysiol.00549.2009
  3. Radius-dependent decline of performance in isolated cardiac muscle does not reflect inadequacy of diffusive oxygen supply
    Han, J.C., Taberner, A.J., Kirton, R.S., Nielsen, P.M., Archer, R., Kim, N., and Loiselle, D.S.
    American Journal of Physiology: Heart and Circulatory Physiology, 2011. 300(4): p. H1222-H1236. 10.1152/ajpheart.01157.2010
  4. Energetics of stress production in isolated cardiac trabeculae from the rat
    Han, J.C., Taberner, A.J., Nielsen, P.M., Kirton, R.S., Ward, M.L., and Loiselle, D.S.
    American Journal of Physiology: Heart and Circulatory Physiology, 2010. 299(5): p. H1382-H1394. 10.1152/ajpheart.00454.2010
  5. Myocardial Twitch Duration and the Dependence of Oxygen Consumption on Pressure-Volume Area: Experiments and Modelling
    Han, J.C., Tran, K., Taberner, A.J., Nickerson, D.P., Kirton, R.S., Nielsen, P.M., Ward, M.L., Nash, M.P., Crampin, E.J., and Loiselle, D.S.
    Journal of Physiology, 2012.
  6. A sensitive flow-through microcalorimeter for measuring the heat production of cardiac trabeculae
    Taberner, A., Kirton, R.S., Loiselle, D., and Hunter, I.
    Conference Proceeding, IEEE Engineering in Medicine and Biology Society, 2004. 3: p. 2030-3.
  7. Stress development, heat production and dynamic modulus of rat isolated cardiac trabeculae revealed in a flow-through micro-mechano-calorimeter
    Taberner, A.J., Han, J.C., Kirton, R.S., Loiselle, D.S., and Nielsen, P.M.
    Conference Proceeding, IEEE Engineering in Medicine and Biology Society, 2010. 2010: p. 1860-3.
  8. A work-loop calorimeter for measuring the force-length-heat relationship of working excised cardiac muscle fibers
    Taberner, A.J., Han, J.C., Loiselle, D.S., and Nielsen, P.M.
    Conf Proc IEEE Eng Med Biol Soc, 2011. 2011: p. 1901-4.
     
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